Concept: Real time biosurveillance using a spooled HEPA capture material. Air is actively drawn through the filter material, capturing slivers in time, where particles including bacteria, fungi, and viruses adhere to the filter. The filter material slowly progresses constantly, so a time stamp can be associated with each detected exposure.
As the HEPA material spool progresses from the aerosol capture element to the biodetection element, a blast of air and liqid releases the captured materials to progress to the automated nucleic acid detection step. In this step, detergents and microsonication break the cells and viral capsids, releasing DNA, RNA, proteins, and other biomaterials. The biomaterials are subjected to a quick charged pulses, attracting and focusing the negatively charged nucleic acids to a positively charged loci point for molecular detection via hybridization. Capture probes to 1,000s of bacterial, viral, and fungal pathogens enable quick scanning for usual suspect pathogens. In cases where an infection exposure is suspected, but no clear pathogen ID has been made, then this sample is reflexed to full metagenomic characterization on the MinION sequencing platform, on the ISS. Data is compared to Genbank , NASA Genelab, and other data repositories to identify potential risk microorganisms and viruses. If a pathogen, or possible pathogen is detected, the Ultra HEPA N99 high airflow purification system is activated, personel notified, and appropriate exposure mitigation immediately put in place.
Technologies used
Discussion